Barr H A, Lugg M A, Nicholas T E
Biol Neonate. 1980;38(3-4):214-20. doi: 10.1159/000241367.
We have used instant thin-layer chromatography and radioimmunoassay to measure the concentrations of total cortisone (E) and cortisol (F) in the fetal and maternal compartments of the rabbit. The concentration of F in fetal blood increased markedly at day 25; this is just prior to the rapid increase in the surfactant phospholipids in lung tissue in this species. This increase coincided with a peak in the concentration of E and F in the maternal blood and may reflect the maternal levels. There was no 'surge' in the concentration of total F in fetal blood at term. The concentration of E in fetal blood varied between 5 and 15 ng/ml during this last stage of gestation, hence providing ready substrate for the 11 beta-reductase present in lung and other tissues. The concentration of E and F in maternal blood was significantly higher and more variable than that in blood from nonpregnant female rabbits, and there was a pronounced peak in concentration at day 30 (term: 31 days). The concentrations of both E and F in amniotic fluid were lower than those in either maternal or fetal blood until day 30 when the concentration of F increased fivefold to 19.2 +/- 2.58 (mean +/- SEM of 6 samples).
我们运用即时薄层色谱法和放射免疫分析法来测定家兔胎儿和母体部分中总可的松（E）和皮质醇（F）的浓度。在第25天，胎儿血液中F的浓度显著升高；这恰好在该物种肺组织中表面活性物质磷脂迅速增加之前。这种增加与母体血液中E和F浓度的峰值相吻合，可能反映了母体水平。足月时胎儿血液中总F的浓度没有“激增”。在妊娠的最后阶段，胎儿血液中E的浓度在5至15纳克/毫升之间变化，因此为肺和其他组织中存在的11β-还原酶提供了现成的底物。母体血液中E和F的浓度显著高于未怀孕雌兔血液中的浓度，且变化更大，在第30天（足月：31天）浓度有一个明显的峰值。直到第30天，羊水内E和F的浓度均低于母体或胎儿血液中的浓度，此时F的浓度增加了五倍，达到19.2±2.58（6个样本的平均值±标准误）。
