嗜热自养甲烷杆菌中甲基辅酶M还原酶系统的ATP激活及其特性
ATP activation and properties of the methyl coenzyme M reductase system in Methanobacterium thermoautotrophicum.
作者信息
Gunsalus R P, Wolfe R S
出版信息
J Bacteriol. 1978 Sep;135(3):851-7. doi: 10.1128/jb.135.3.851-857.1978.
Abstract
The requirement of ATP for the methyl coenzyme M methylreductase in extracts of Methanobacterium thermoautotrophicum was found to be catalytic; for each mol of ATP added, 15 mol of methane was produced from methyl coenzyme M [2-(methylthio)ethanesulfonic acid]. Other nucleotide triphosphates partially replaced ATP in activation of the reductase. All components of the reaction were found in the supernatant fraction of cell extracts after centrifugation at 100,000 X g for 1 h; optimal reaction rates occurred at 65 degrees C, at a pH range of 5.6 to 6.0, and at concentrations of ATP and MgCl2 of 1 mM and 40 mM, respectively. Chloral hydrate, chloroform, nitrite, 2,4-dinitrophenol, and viologen dyes (compounds known to inhibit methanogenesis from a variety of substrates) were found to inhibit the conversion of methyl coenzyme M to methane. Methyl coenzyme M methylreductase was shown to be present in a variety of methanogens.
摘要
研究发现，嗜热自养甲烷杆菌提取物中甲基辅酶M甲基还原酶对ATP的需求具有催化作用；每添加1摩尔ATP，可从甲基辅酶M[2-(甲硫基)乙烷磺酸]产生15摩尔甲烷。其他核苷三磷酸在一定程度上可替代ATP激活该还原酶。将细胞提取物在100,000×g离心1小时后，反应的所有成分均存在于上清液部分；最佳反应速率出现在65℃、pH值为5.6至6.0、ATP和MgCl2浓度分别为1 mM和40 mM的条件下。发现水合氯醛、氯仿、亚硝酸盐、2,4-二硝基苯酚和紫精染料（已知可抑制多种底物产甲烷的化合物）可抑制甲基辅酶M向甲烷的转化。甲基辅酶M甲基还原酶存在于多种产甲烷菌中。
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