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鉴定结肠粘蛋白中O-酰化唾液酸的特定技术。

Specific techniques for the identification of O-acylated sialic acids in colonic mucins.

作者信息

Culling C F, Reid P E

出版信息

J Microsc. 1980 Aug;119(3):415-25. doi: 10.1111/j.1365-2818.1980.tb04113.x.

DOI:10.1111/j.1365-2818.1980.tb04113.x
PMID:7411601
Abstract

The development of methodology for the histochemistry of mucins based upon their PAS reactivity is discussed in terms of mechanism, specificity and application. Two new histochemical methods (PB/KOH/PAS and PAT/KOH/PAS), supplemented by a variety of new and standard histochemical techniques, and correlated by parallel chemical studies, were used to demonstrate and identify C4 and side chain O-acylated sialic acids in colonic epithelial mucins. The application of these methods in the field of histopathology is discussed.

摘要

本文从机制、特异性和应用方面讨论了基于黏蛋白过碘酸希夫反应（PAS反应）的组织化学方法的发展。两种新的组织化学方法（PB/KOH/PAS和PAT/KOH/PAS），辅以多种新的和标准的组织化学技术，并通过平行化学研究进行关联，用于证明和鉴定结肠上皮黏蛋白中的C4和侧链O-酰化唾液酸。本文还讨论了这些方法在组织病理学领域的应用。

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Specific techniques for the identification of O-acylated sialic acids in colonic mucins.鉴定结肠粘蛋白中O-酰化唾液酸的特定技术。
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