Culling C F, Reid P E, Dunn W L
J Clin Pathol. 1979 Dec;32(12):1272-7. doi: 10.1136/jcp.32.12.1272.
Two histochemical techniques, the PAT/KOH/PAS and the PBT/KOH/PAS, were used to investigate the side chain O-acyl substitution patterns of the sialic acids of the colonic epithelial mucins in cases of ulcerative colitis and Crohn's disease. In both diseases there was, as compared to normal, a reduction in the proportion of sialic acids O-acylated at C7C8, the reduction being greater in ulcerative colitis. Further, there appeared to be an association between the severity of the disease and the reduction in the staining of O-acylated sialic acids. This relationship was more marked in ulcerative colitis. In some cases of both diseases there was evidence for epithelial mucins containing predominantly C7-substituted sialic acids. This study has confirmed our previous conclusion that, in Crohn's disease of the terminal ileum, the disease is associated with an increase in the proportion of sialic acids bearing side chain substituents.
采用两种组织化学技术，即PAT/KOH/PAS和PBT/KOH/PAS，来研究溃疡性结肠炎和克罗恩病患者结肠上皮黏蛋白中唾液酸的侧链O-酰基取代模式。与正常情况相比，在这两种疾病中，C7C8位O-酰化唾液酸的比例均降低，溃疡性结肠炎中的降低更为明显。此外，疾病的严重程度与O-酰化唾液酸染色的降低之间似乎存在关联。这种关系在溃疡性结肠炎中更为显著。在这两种疾病的某些病例中，有证据表明上皮黏蛋白主要含有C7位取代的唾液酸。本研究证实了我们之前的结论，即在回肠末端克罗恩病中，该疾病与带有侧链取代基的唾液酸比例增加有关。
