Gooneratne S R, Howell J M
Res Vet Sci. 1980 May;28(3):351-61.
Chronic copper poisoning was induced in sheep by repeated dosing with an aqueous solution of copper sulphate. A sudden rise in the level of plasma creatine kinase (CK) occurred at haemolysis but the level returned to normal once the 'crisis' was over. The level of CK in the cerebrospinal fluid remained normal throughout the experiments. An isoenzyme study showed that the CK was liberated from muscle. Light microscopic studies failed to reveal consistent changes in muscle structure but ultrastructural changes in mitochondria, similar to those described as the earlies changes in muscle fibres in vitamin E deficiency, were seen in sheep killed during and after haemolysis. The elevations of CK levels and the mitochondrial changes were most marked in sheep that did not receive selenium and vitamin E supplements. It is suggested that the CK release at haemolysis may occur because of a transient increase in the permeability of muscle membranes which may be brought about by a number of factors such as: (a) hypoxia, (b) hypercupraemia, (c) decrease in vitamin E and/or selenium content in blood and tissues.
通过反复给绵羊投喂硫酸铜水溶液诱导其发生慢性铜中毒。溶血时血浆肌酸激酶（CK）水平突然升高，但“危机”结束后该水平恢复正常。在整个实验过程中，脑脊液中的CK水平保持正常。同工酶研究表明，CK是从肌肉中释放出来的。光学显微镜研究未能发现肌肉结构有一致的变化，但在溶血期间及之后处死的绵羊中，观察到线粒体的超微结构变化，类似于维生素E缺乏时肌肉纤维最早出现的变化。在未补充硒和维生素E的绵羊中，CK水平的升高和线粒体变化最为明显。有人认为，溶血时CK的释放可能是由于肌肉膜通透性的短暂增加所致，这可能由多种因素引起，如：（a）缺氧，（b）高铜血症，（c）血液和组织中维生素E和/或硒含量降低。
