Halter S A, Greene H L, Helinek G
Scan Electron Microsc. 1980(3):155-61.
Sequential small intestinal biopsies from patients with gluten sensitive enteropathy were studied by scanning electron microscopy before and after dietary treatment. Compared with patients without evidence of villus atrophy, there were marked architectural changes. Before treatment, villi were absent and crypts were prominent. After treatment, the intestinal repair could be divided into stages. The first stage consisted of circular ridges surrounding the crypts. Unequal cellular repletion around the crypts resulted in the formation of horseshoe shaped ridges. Elongation of the arms of the horseshoes led to the formation of cerebriform ridges. Mitten-like villi were formed in the last stage by the development of furrows and septation of the ridges. The scanning electron microscope is a useful adjunct to the light microscopic and dissecting microscopic assessment of small intestinal biopsies. It can detect changes not possible with either light microscopy or the dissecting microscope and may be a more sensitive technique for the morphological assessment of patient response to treatment for gluten sensitive enteropathy.
对麸质敏感肠病患者进行饮食治疗前后，通过扫描电子显微镜对其小肠活检标本进行了连续研究。与无绒毛萎缩迹象的患者相比，存在明显的结构变化。治疗前，绒毛缺失，隐窝突出。治疗后，肠道修复可分为几个阶段。第一阶段由围绕隐窝的环形嵴组成。隐窝周围细胞填充不均导致形成马蹄形嵴。马蹄形臂的伸长导致脑回样嵴的形成。在最后阶段，通过沟的形成和嵴的分隔形成手套样绒毛。扫描电子显微镜是对小肠活检进行光学显微镜和解剖显微镜评估的有用辅助手段。它可以检测到光学显微镜或解剖显微镜无法检测到的变化，并且可能是评估麸质敏感肠病患者对治疗反应的形态学更敏感的技术。
