Garfia A, Mestres P, Rascher K
Scan Electron Microsc. 1980(3):449-56.
These experiments were performed in order to study the ventricular walls of the brain following more or less extensive lesioning of the ependyma and during the process of repair. Trinitrophenol (TNP, 1% in saline) was injected into the ventricular system of adult rats of both sexes. The animals were fixed for SEM/TEM 26th or 5, 7, 11, 21, 30 and 40d post injection. TNP caused areas of irreversible lesions in ependymal cells but the surrounding unlesioned cells showed no signs of a regenerative reaction. The number of supraependymal cells increased considerably at the sites of lesion, their shapes being markedly different from those of comparable cells in control animals. The subependymal tissue presented spongiosis. After a phase in which the lesioned ventricular surface was covered with abundant cell debris, the subependymal astroglia cell debris, the subependymal astroglia proliferated, producing an extensive layer of cell processes which substituted for the missing ependymal cells. This investigation has given us the opportunity to study the formation and morphological characteristics, particularly as visualized with the SEM, of an experimentally induced gliosis in the ventricular wall of adult rats.
进行这些实验是为了研究在室管膜或多或少广泛受损后以及修复过程中大脑的心室壁。将三硝基苯酚（TNP，盐水中1%）注入成年雌雄大鼠的脑室系统。在注射后第26天或5、7、11、21、30和40天对动物进行固定以用于扫描电子显微镜/透射电子显微镜观察。TNP导致室管膜细胞出现不可逆损伤区域，但周围未受损的细胞没有显示出再生反应的迹象。在损伤部位，室管膜上细胞的数量显著增加，其形状与对照动物中类似细胞的形状明显不同。室管膜下组织出现海绵样变。在受损的心室表面覆盖有大量细胞碎片的阶段之后，室管膜下星形胶质细胞增殖，产生一层广泛的细胞突起，替代缺失的室管膜细胞。这项研究让我们有机会研究成年大鼠心室壁实验性诱导胶质增生的形成和形态特征，特别是通过扫描电子显微镜观察到的特征。
