Armond P A, Björkman O, Staehelin L A
Biochim Biophys Acta. 1980 Oct 2;601(3):433-43. doi: 10.1016/0005-2736(80)90547-7.
High temperature-induced alterations to membrane structure were investigated for chloroplast thylakoid membranes isolated from leaves of Nerium oleander grown at a 20/15 degrees C or 45/32 degrees C day/night temperature regime and pretreated at temperatures from 40 to 55 degrees C. Quantitative analysis of micrographs of freeze-fractured membranes revealed a progressive loss of exoplasmic fracture face (EF) particles from the larger particle size classes as the temperature of the pretreatment was increased. This loss indicates that the components of the EF particles, presumed to be the chlorophyll a/b light-harvesting complex and the photosystem II core complex become physically dissociated as a result of the heat pretreatment. The high-temperature stability of this supramolecular complex is enhanced in the samples from the plants grown at the higher temperature regime. These results demonstrate that the heat-induced damage to the photosynthetic apparatus involves not only a functional dissociation of the chlorophyll a/b light-harvesting complex from the photosystem Ii complex, but a physical dissociation as well.
研究了高温对从夹竹桃叶片分离的叶绿体类囊体膜结构的影响，这些夹竹桃叶片分别在20/15摄氏度或45/32摄氏度的日/夜温度条件下生长，并在40至55摄氏度的温度下进行预处理。对冷冻断裂膜的显微照片进行定量分析发现，随着预处理温度的升高，较大粒径类别的外质断裂面（EF）颗粒逐渐减少。这种减少表明，推测为叶绿素a/b捕光复合体和光系统II核心复合体的EF颗粒成分由于热预处理而发生物理解离。在较高温度条件下生长的植物样品中，这种超分子复合体的高温稳定性增强。这些结果表明，热诱导对光合机构的损伤不仅涉及叶绿素a/b捕光复合体与光系统II复合体的功能解离，还涉及物理解离。
