The determination of lecithin:cholesterol acyl transferase in the clinical laboratory: a modified enzymatic procedure.

A very simple and fast method for the determination of lecithin:cholesterol acyltransferase is described. The method is based on the enzymatic determination of free cholesterol in a serum or plasma sample before and after 40 min incubation at 37 degrees C. The proposed procedure is particularly useful for routine application in a clinical laboratory. It works with a coefficient of variation of less than 5%.