Ghory A C, Patterson R, Roberts M, Suszko I
Clin Exp Immunol. 1980 Jun;40(3):581-5.
Peripheral blood lymphocytes from five normal donors and five individuals with allergic bronchopulmonary aspergillosis (ABPA) were used for measurement of in vitro IgE formation in tissue culture preparations. IgE was measured by a sensitive radioimmunoassay using concentrated tissue culture medium (TCM) and expressed as ng IgE/ml of TCM. The results demonstrated that IgE was formed by unstimulated lymphocytes from one of five normal donors (range 1.5-2.4 ng/ml) and in two of five ABPA donors (range 2-4 ng/ml). However, when different dilutions of pokeweed mitogen were added to the tissue culture, three of five normal donors and three of five ABPA donors in remission showed enhanced IgE formation. At the time of an ABPA exacerbation in one individual, there was a definite increse in IgE synthesis up to 40 ng/ml which was suppressed by pokeweed mitogen to 17.3 ng/ml. Clear differences between normals and ABPA patients in remission are not apparent.
来自5名正常供体和5名变应性支气管肺曲霉病（ABPA）患者的外周血淋巴细胞被用于在组织培养制剂中测量体外IgE的形成。使用浓缩组织培养基（TCM）通过灵敏的放射免疫测定法测量IgE，并表示为每毫升TCM中IgE的纳克数。结果表明，来自5名正常供体中的1名（范围为1.5 - 2.4纳克/毫升）以及5名ABPA供体中的2名（范围为2 - 4纳克/毫升）的未刺激淋巴细胞形成了IgE。然而，当将不同稀释度的商陆有丝分裂原添加到组织培养中时，5名正常供体中的3名以及5名处于缓解期的ABPA供体中的3名显示出IgE形成增加。在1名个体的ABPA加重期，IgE合成明显增加至40纳克/毫升，而被商陆有丝分裂原抑制至17.3纳克/毫升。正常人与处于缓解期的ABPA患者之间的明显差异并不显著。
