• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

商陆丝裂原与氯化汞协同作用在体外选择性增强人IgE的产生。

Selective enhancement of human IgE production in vitro by synergy of pokeweed mitogen and mercuric chloride.

作者信息

Kimata H, Shinomiya K, Mikawa H

出版信息

Clin Exp Immunol. 1983 Jul;53(1):183-91.

PMID:6223732
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1535527/
Abstract

IgE production in vitro was investigated in cultures of human peripheral blood mononuclear cells (MNC) from non-atopic donors with pokeweed mitogen (PWM), mercuric chloride (HgCl2), or both. PWM alone induced a few IgE immunoglobulin secreting cells (ISC) detected by reverse plaque forming cells (PFC) and many IgG, IgM, and IgA PFC. HgCl2 alone failed to produce significant numbers of ISC of any class. PWM plus HgCl2 caused a selective increase of IgE PFC without affecting IgG, IgM, and IgA PFC. Co-cultures of B cells plus mitomycin C (MMC) treated T cells stimulated by PWM alone produced more IgG, IgM, IgA and IgE PFC than those of B cells plus T cells. However, PWM plus HgCl2 produced significantly more IgE PFC selectively in those cultures. This effect was observed in the cells of most of the donors, but a few donors showed different responses.

摘要

在来自非特应性供体的人外周血单个核细胞（MNC）培养物中，使用商陆有丝分裂原（PWM）、氯化汞（HgCl2）或两者来研究体外IgE的产生。单独使用PWM可诱导通过反向空斑形成细胞（PFC）检测到的少量IgE免疫球蛋白分泌细胞（ISC）以及许多IgG、IgM和IgA PFC。单独使用HgCl2未能产生任何类别的大量ISC。PWM加HgCl2导致IgE PFC选择性增加，而不影响IgG、IgM和IgA PFC。单独由PWM刺激的B细胞加丝裂霉素C（MMC）处理的T细胞的共培养物比B细胞加T细胞的共培养物产生更多的IgG、IgM、IgA和IgE PFC。然而，PWM加HgCl2在那些培养物中选择性地产生了明显更多的IgE PFC。在大多数供体的细胞中观察到了这种效应，但少数供体表现出不同的反应。

相似文献

1
Selective enhancement of human IgE production in vitro by synergy of pokeweed mitogen and mercuric chloride.商陆丝裂原与氯化汞协同作用在体外选择性增强人IgE的产生。
Clin Exp Immunol. 1983 Jul;53(1):183-91.
2
Induction of human immunoglobulin secretion. I. Synergistic effect of B cell mitogen Cowan I plus T cell mitogens or factors.人免疫球蛋白分泌的诱导。I. B细胞促有丝分裂原考恩I与T细胞促有丝分裂原或因子的协同作用。
J Immunol. 1981 Sep;127(3):1044-7.
3
Clinical evaluation of B cell and T-regulator cell function using a protein A haemolytic plaque assay.使用蛋白A溶血空斑试验对B细胞和T调节细胞功能进行临床评估。
Clin Exp Immunol. 1981 Oct;46(1):196-205.
4
Recombinant human IL-4 induces IgE and IgG synthesis by normal and atopic donor mononuclear cells. Similar dose response, time course, requirement for T cells, and effect of pokeweed mitogen.重组人白细胞介素-4可诱导正常供体和特应性供体的单核细胞合成IgE和IgG。类似的剂量反应、时间进程、对T细胞的需求以及商陆有丝分裂原的作用。
J Immunol. 1990 Mar 15;144(6):2123-30.
5
Circulating and pokeweed mitogen-induced immunoglobulin-secreting cells in systemic lupus erythematosus.系统性红斑狼疮中循环及商陆有丝分裂原诱导的免疫球蛋白分泌细胞
Clin Exp Immunol. 1979 Jan;35(1):76-88.
6
Activation of human B lymphocytes. XV. Spontaneously occurring and mitogen-induced indirect anti-sheep red blood cell plaque-forming cells in normal human peripheral blood.人类B淋巴细胞的激活。十五、正常人外周血中自发产生的和丝裂原诱导的间接抗绵羊红细胞空斑形成细胞
J Immunol. 1980 May;124(5):2410-3.
7
Human IgE antibody synthesis in vitro: stimulation of IgE responses by pokeweed mitogen and selective inhibition of such responses by human suppressive factor of allergy (SFA).人IgE抗体的体外合成：商陆有丝分裂原对IgE反应的刺激以及人变应性抑制因子（SFA）对这种反应的选择性抑制。
J Immunol. 1981 Sep;127(3):1169-77.
8
Pokeweed mitogen induced synthesis of human IgG and IgM in vitro. Technical aspects and the role of adherent cells.商陆丝裂原在体外诱导人IgG和IgM的合成。技术方面及贴壁细胞的作用。
Biomed Biochim Acta. 1986;45(4):467-76.
9
Development of IgE-forming cells in vitro from rat mesenteric lymph node cells.大鼠肠系膜淋巴结细胞体外IgE形成细胞的发育
J Immunol. 1978 Dec;121(6):2413-21.
10
Pokeweed mitogen induced differentiation of human B cells: evaluation by a protein A haemolytic plaque assay.商陆有丝分裂原诱导人B细胞分化：用蛋白A溶血空斑试验进行评估
Immunology. 1979 Sep;38(1):181-9.

引用本文的文献

1
Metals and kidney autoimmunity.金属与肾脏自身免疫
Environ Health Perspect. 1999 Oct;107 Suppl 5(Suppl 5):753-65. doi: 10.1289/ehp.99107s5753.
2
Mercuric chloride, a chemical responsible for T helper cell (Th)2-mediated autoimmunity in brown Norway rats, directly triggers T cells to produce interleukin-4.氯化汞是导致棕色挪威大鼠中辅助性T细胞（Th）2介导的自身免疫的一种化学物质，它直接触发T细胞产生白细胞介素-4。
J Clin Invest. 1995 Sep;96(3):1484-9. doi: 10.1172/JCI118185.
3
Interleukin-2 production in Brown-Norway rats with HgCl2-induced autoimmune disease: paradoxical in vivo versus in vitro findings.氯化汞诱导的自身免疫性疾病棕色挪威大鼠中白细胞介素-2的产生：体内与体外研究结果的矛盾之处
Clin Exp Immunol. 1988 Sep;73(3):401-5.
4
HgC12 induces T and B cells to proliferate and differentiate in BN rats.氯化汞诱导BN大鼠的T细胞和B细胞增殖与分化。
Clin Exp Immunol. 1988 Feb;71(2):336-42.
5
Immunotoxicology: suppressive and stimulatory effects of drugs and environmental chemicals on the immune system. A discussion.免疫毒理学：药物和环境化学物质对免疫系统的抑制和刺激作用。讨论
Arch Toxicol. 1989;63(4):257-73. doi: 10.1007/BF00278639.

本文引用的文献

1
In vitro IgE formation by peripheral blood lymphocytes from normal individuals and patients with allergic bronchopulmonary aspergillosis.正常个体及变应性支气管肺曲霉病患者外周血淋巴细胞的体外IgE形成
Clin Exp Immunol. 1980 Jun;40(3):581-5.
2
In vitro synthesis of IgE by human peripheral blood leucocytes: relationship to serum titre of leucocyte donor.人外周血白细胞体外合成IgE：与白细胞供体血清滴度的关系。
Clin Exp Immunol. 1981 Mar;43(3):458-62.
3
Human IgE synthesis in vitro: a reassessment.体外人IgE合成：重新评估
J Immunol. 1981 Sep;127(3):829-34.
4
Human IgE antibody synthesis in vitro: stimulation of IgE responses by pokeweed mitogen and selective inhibition of such responses by human suppressive factor of allergy (SFA).人IgE抗体的体外合成：商陆有丝分裂原对IgE反应的刺激以及人变应性抑制因子（SFA）对这种反应的选择性抑制。
J Immunol. 1981 Sep;127(3):1169-77.
5
Stimulation of primary in vitro IgE antibody responses in culture of human peripheral mononuclear cells.人外周血单个核细胞培养中初级体外IgE抗体反应的刺激
J Exp Med. 1981 Jun 1;153(6):1574-81. doi: 10.1084/jem.153.6.1574.
6
In vitro production of IgE by human peripheral blood lymphocytes. Effect of serum from patients with Kimura's disease.
Arerugi. 1982;31(7):417-24.
7
Human B cell function in normal individuals of various ages. 1. In vitro enumeration of pokeweed-induced peripheral blood lymphocyte immunoglobulin-synthesizing cells and the comparison of the results with numbers of peripheral B and T cells, mitogen responses, and levels of serum immunoglobulins.不同年龄正常个体的人类B细胞功能。1. 体外计数商陆诱导的外周血淋巴细胞免疫球蛋白合成细胞，并将结果与外周B细胞和T细胞数量、丝裂原反应以及血清免疫球蛋白水平进行比较。
Clin Exp Immunol. 1981 Jun;44(3):646-53.
8
Induction of IgE synthesis and potentiation of anti-ovalbumin IgE antibody response by HgCl2 in the rat.氯化汞对大鼠IgE合成的诱导作用及抗卵清蛋白IgE抗体反应的增强作用
J Immunol. 1981 Feb;126(2):699-792.
9
Evaluation of IgM, IgG, IgA, IgD, and IgE secretion by human peripheral blood lymphocytes in cultures stimulated with pokeweed mitogen and Staphylococcus aureus Cowan I.用美洲商陆丝裂原和金黄色葡萄球菌Cowan I刺激培养的人外周血淋巴细胞，对其分泌的IgM、IgG、IgA、IgD和IgE进行评估。
Cell Immunol. 1980 Mar 1;50(1):115-24. doi: 10.1016/0008-8749(80)90011-8.
10
In vitro production of IgE by human peripheral blood mononuclear cells. IV. Modulation by allergen of the spontaneous IgE antibody biosynthesis.人外周血单个核细胞体外产生IgE。IV. 变应原对自发性IgE抗体生物合成的调节作用
Clin Exp Immunol. 1982 Jul;49(1):185-92.