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商陆丝裂原与氯化汞协同作用在体外选择性增强人IgE的产生。

Selective enhancement of human IgE production in vitro by synergy of pokeweed mitogen and mercuric chloride.

作者信息

Kimata H, Shinomiya K, Mikawa H

出版信息

Clin Exp Immunol. 1983 Jul;53(1):183-91.

PMID:6223732
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1535527/
Abstract

IgE production in vitro was investigated in cultures of human peripheral blood mononuclear cells (MNC) from non-atopic donors with pokeweed mitogen (PWM), mercuric chloride (HgCl2), or both. PWM alone induced a few IgE immunoglobulin secreting cells (ISC) detected by reverse plaque forming cells (PFC) and many IgG, IgM, and IgA PFC. HgCl2 alone failed to produce significant numbers of ISC of any class. PWM plus HgCl2 caused a selective increase of IgE PFC without affecting IgG, IgM, and IgA PFC. Co-cultures of B cells plus mitomycin C (MMC) treated T cells stimulated by PWM alone produced more IgG, IgM, IgA and IgE PFC than those of B cells plus T cells. However, PWM plus HgCl2 produced significantly more IgE PFC selectively in those cultures. This effect was observed in the cells of most of the donors, but a few donors showed different responses.

摘要

在来自非特应性供体的人外周血单个核细胞（MNC）培养物中，使用商陆有丝分裂原（PWM）、氯化汞（HgCl2）或两者来研究体外IgE的产生。单独使用PWM可诱导通过反向空斑形成细胞（PFC）检测到的少量IgE免疫球蛋白分泌细胞（ISC）以及许多IgG、IgM和IgA PFC。单独使用HgCl2未能产生任何类别的大量ISC。PWM加HgCl2导致IgE PFC选择性增加，而不影响IgG、IgM和IgA PFC。单独由PWM刺激的B细胞加丝裂霉素C（MMC）处理的T细胞的共培养物比B细胞加T细胞的共培养物产生更多的IgG、IgM、IgA和IgE PFC。然而，PWM加HgCl2在那些培养物中选择性地产生了明显更多的IgE PFC。在大多数供体的细胞中观察到了这种效应，但少数供体表现出不同的反应。

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Human IgE antibody synthesis in vitro: stimulation of IgE responses by pokeweed mitogen and selective inhibition of such responses by human suppressive factor of allergy (SFA).人IgE抗体的体外合成：商陆有丝分裂原对IgE反应的刺激以及人变应性抑制因子（SFA）对这种反应的选择性抑制。
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In vitro production of IgE by human peripheral blood lymphocytes. Effect of serum from patients with Kimura's disease.
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