Namba M, Nishitani K, Kimoto T
Gan. 1980 Jun;71(3):300-7.
The characteristics of WI-38 CT-1 cells, which had been transformed by treatment with Co-60 gamma rays, were compared with those of the control WI-38 cells in order to identify parameters of malignant transformation in cultures of normal human diploid cells. The transformed WI-38 CT-1 cells were different from the control WI-38 cells in the following respects: (1) epithelial-like morphology of the cells, (2) reduced serum requirement for cell proliferation, (3) increased colony formation in soft agar, (4) increased saturation density, and (5) resistance to the cytostatic effect of theophylline on cell proliferation. On the other hand, no appreciable difference was detected between the control and the transformed cells in (1) population doubling time and (2) sensitivity to Co-60 gamma rays.
将经钴 - 60γ射线处理转化后的WI - 38 CT - 1细胞的特性与对照WI - 38细胞的特性进行比较，以确定正常人二倍体细胞培养中恶性转化的参数。转化后的WI - 38 CT - 1细胞在以下方面与对照WI - 38细胞不同：（1）细胞呈上皮样形态，（2）细胞增殖所需血清减少，（3）软琼脂中集落形成增加，（4）饱和密度增加，（5）对茶碱对细胞增殖的细胞抑制作用具有抗性。另一方面，在（1）群体倍增时间和（2）对钴 - 60γ射线的敏感性方面，未检测到对照细胞与转化细胞之间有明显差异。
