Lonovics J, Guzman S, Devitt P, Hejtmancik K E, Suddith R L, Rayford P L, Thompson J C
Gastroenterology. 1980 Nov;79(5 Pt 1):817-22.
Plasma levels of pancreatic polypeptide were measured after a test meal and after infusion of graded doses of 99% pure cholecystokinin in 6 healthy volunteers. Initial attempts at sterilization of 99% pure cholecystokinin resulted in complete inactivation. Successful sterilization was accomplished by filtration by using specially treated silver-coated filters. Biologic activity of sterilized material was confirmed with cholecystokinin bioassay, and plasma levels of sterilized cholecystokinin achieved by exogenous infusions were measured with a specific cholecystokinin radioimmunoassay. Significant increases in plasma levels of pancreatic polypeptide were found with a test meal and with infusions of 0.25 and 0.5 micrograms/kg-hr of 99% pure cholecystokinin. Integrated values of pancreatic polypeptide released by the low-dose and the high-dose infusions of 99% pure cholecystokinin were 59% and 50% of that obtained by food, respectively. Integrated levels of cholecystokinin after 45 min of infusion of 0.25 micrograms/kg-hr were equal to those after a standard meal. Cholecystokinin, therefore, is an effective humoral releaser of pancreatic polypeptide in humans and may play an important role in the intestinal phase of release of pancreatic polypeptide.
在6名健康志愿者中，于试餐后及输注不同剂量的99%纯胆囊收缩素后，测定了血浆中胰多肽的水平。最初对99%纯胆囊收缩素进行灭菌的尝试导致其完全失活。通过使用经过特殊处理的镀银滤器进行过滤，成功实现了灭菌。用胆囊收缩素生物测定法证实了灭菌材料的生物活性，并用特异性胆囊收缩素放射免疫测定法测量了通过外源性输注达到的灭菌胆囊收缩素的血浆水平。发现试餐以及输注0.25和0.5微克/千克·小时的99%纯胆囊收缩素后，血浆中胰多肽水平显著升高。99%纯胆囊收缩素低剂量和高剂量输注所释放的胰多肽的积分值分别为食物所诱导值的59%和50%。输注0.25微克/千克·小时45分钟后的胆囊收缩素积分水平与标准餐后的水平相当。因此，胆囊收缩素是人类胰多肽的一种有效的体液释放因子，可能在胰多肽释放的肠期发挥重要作用。
