Kawaguchi A, Arai K, Seyama Y, Yamakawa T, Okuda S
J Biochem. 1980 Aug;88(2):303-6. doi: 10.1093/oxfordjournals.jbchem.a132975.
The pattern of fatty acids produced by the fatty acid synthetase complex of Brevibacterium ammoniagenes under several conditions was examined. The fatty acid synthetase obtained from B. ammoniagenes produced oleic acid as well as saturated fatty acids (palmitic and stearic acids). The relative proportions of palmitic to stearic acids varied over a wide range. Such alterations were dependent on the malonyl-CoA concentration and the ratio of acetyl-CoA to malonyl-CoA concentrations. At malonyl-CoA concentrations higher than 100 microM, stearic acid accounted for more than 90% of the saturated fatty acids and the pattern of fatty acid synthesized was independent on the ratio of acetyl-CoA to malonyl-CoA. At malonyl-CoA concentrations lower than 100 microM, raising the acetyl-CoA/malonyl-CoA ratio increased the percentage of palmitic acid. However, the proportion of oleic acid produced remained almost constant under all conditions tested.
考察了产氨短杆菌脂肪酸合成酶复合体在几种条件下产生的脂肪酸模式。从产氨短杆菌获得的脂肪酸合成酶产生油酸以及饱和脂肪酸（棕榈酸和硬脂酸）。棕榈酸与硬脂酸的相对比例在很宽的范围内变化。这种变化取决于丙二酸单酰辅酶A浓度以及乙酰辅酶A与丙二酸单酰辅酶A浓度的比例。在丙二酸单酰辅酶A浓度高于100微摩尔时，硬脂酸占饱和脂肪酸的90%以上，合成的脂肪酸模式与乙酰辅酶A与丙二酸单酰辅酶A的比例无关。在丙二酸单酰辅酶A浓度低于100微摩尔时，提高乙酰辅酶A/丙二酸单酰辅酶A比例会增加棕榈酸的百分比。然而，在所有测试条件下产生的油酸比例几乎保持恒定。
