Chanas A C, Young P R, Ellis D S, Mann G, Stamford S, Howard C R
Arch Virol. 1980;65(2):157-67. doi: 10.1007/BF01317327.
The reaction between Pichinde virus and homologous antisera has been studied using a plaque size reduction method. The incorporation of antiserum in the overlay of infected Vero cell monolayers revealed a pattern of virus-cell interactions which were manifested by both a significant reduction in the diameter of virus plaques, and regeneration of cells in the centre of each. Electron microscopy demonstrated that antibody molecules were bound to virus particles budding from the surface of infected cells resulting in the formation of extracellular virus-antibody complexes. These aggregates were subsequently detected in vacuoles of freshly-infected cells. In the absence of virus neutralization, reaction of Pichinde virus with homologous antiserum leads to the formation of infectious aggregates which due to their larger size restrict the rate of plaque development.
已使用蚀斑大小减小法研究了皮钦德病毒与同源抗血清之间的反应。在感染的非洲绿猴肾细胞单层的覆盖物中加入抗血清，揭示了一种病毒-细胞相互作用模式，其表现为病毒蚀斑直径显著减小，且每个蚀斑中心的细胞再生。电子显微镜显示抗体分子与从受感染细胞表面芽生的病毒颗粒结合，导致形成细胞外病毒-抗体复合物。这些聚集体随后在新感染细胞的液泡中被检测到。在没有病毒中和的情况下，皮钦德病毒与同源抗血清的反应导致形成感染性聚集体，由于其较大的尺寸限制了蚀斑形成的速率。
