细菌中性蛋白酶用于小鼠肿瘤和多细胞肿瘤球体的解离。
Use of bacterial neutral protease for disaggregation of mouse tumours and multicellular tumor spheroids.
作者信息
Twentyman P R, Yuhas J M
出版信息
Cancer Lett. 1980 May;9(3):225-8. doi: 10.1016/0304-3835(80)90091-9.
PMID:7013975
Abstract
A bacterial neutral protease (from Bacillus Polymixa) has been used to disaggregate both solid mouse tumours and multicellular tumour spheroids. The cell yields obtained were much higher than those obtained using trypsin whilst the plating efficiency of the resultant single cells were comparable. The use of this neutral protease is simple, inexpensive and highly efficient.
摘要
一种细菌中性蛋白酶（来自多粘芽孢杆菌）已被用于分解实体小鼠肿瘤和多细胞肿瘤球体。获得的细胞产量远高于使用胰蛋白酶时获得的产量，而所得单细胞的接种效率相当。这种中性蛋白酶的使用简单、廉价且高效。
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