Kim Y S, Jatoi I, Kim Y
Diabetologia. 1980 May;18(5):407-11. doi: 10.1007/BF00276822.
In order to determine the effect of maternal diabetes on the somatic growth of the rat fetus and to elucidate mechanisms underlying the control of fetal growth, concentrations of DNA and proteins and DNA polymerase-alpha activities in neonates were examined. The maternal status was classified as normal (no urinary glucose excretion), mildly diabetic (0.01-0.99 g/day urinary glucose), and severely diabetic (1.00 g/day or more urinary glucose). The total DNA contents in mg/neonate were 26.8 +/- 2.2 (mean +/- SEM), 31.3 +/- 2.5, and 29.4 +/- 2.7 for neonates from normal, mildly diabetic and severely diabetic mothers, respectively. The DNA polymerase activities in (cpm/g neonate) X 10(-3) for the same groups of neonates were 432 +/- 58, 1,008 +/- 74, and 888 +/- 118, respectively. These results indicate that the neonatal macrosomia disappears as the severity of maternal diabetes increases. Furthermore, DNA polymerase is one of possible biochemical sites through which macrosomia is manifested in diabetic pregnancies.
为了确定母体糖尿病对大鼠胎儿躯体生长的影响，并阐明胎儿生长控制的潜在机制，对新生儿的DNA和蛋白质浓度以及DNA聚合酶α活性进行了检测。母体状态分为正常（无尿糖排泄）、轻度糖尿病（每日尿糖排泄量为0.01 - 0.99克）和重度糖尿病（每日尿糖排泄量为1.00克或更多）。正常、轻度糖尿病和重度糖尿病母亲所生新生儿的总DNA含量（毫克/新生儿）分别为26.8±2.2（平均值±标准误）、31.3±2.5和29.4±2.7。同一组新生儿的DNA聚合酶活性（每分钟计数/克新生儿）×10⁻³分别为432±58、1008±74和888±118。这些结果表明，随着母体糖尿病严重程度的增加，新生儿巨大儿现象消失。此外，DNA聚合酶是糖尿病妊娠中出现巨大儿现象的可能生化位点之一。
