Hill D J, Milner R D
Diabetologia. 1980 Aug;19(2):143-7. doi: 10.1007/BF00421861.
The effect of insulin injection in fetal rabbits on plasma somatomedin activity and cartilage metabolism was investigated. One fetus in each of 12 litters was injected with 1 unit of insulin zinc suspension subcutaneously on day 27 of gestation and a control fetus was injected with the same volume of 0.154 mol/l saline. The litter was delivered by caesarean section on day 29 and each fetus identified. Plasma somatomedin activity was determined by fetal rabbit cartilage bioassay. Costal cartilage from individual fetuses was incubated in medium containing [3H]thymidine or [35S]sulphate as indicators of cell replication and matrix synthesis respectively. Individual values for somatomedin activity or cartilage isotope uptake were ranked within a litter. In each case the rank in the litter of the insulin-injected fetus, but not the saline-injected fetus, was significantly higher than the mean rank of the litter. Insulin did not stimulate cartilage metabolism in vitro.
研究了胰岛素注射对胎兔血浆生长调节素活性和软骨代谢的影响。在妊娠第27天，对12窝胎兔中的每窝一只胎儿皮下注射1单位胰岛素锌混悬液，另一只对照胎儿注射相同体积的0.154mol/l生理盐水。在第29天通过剖腹产取出胎兔，识别每只胎儿。通过胎兔软骨生物测定法测定血浆生长调节素活性。将单个胎儿的肋软骨分别在含有[3H]胸腺嘧啶核苷或[35S]硫酸盐的培养基中孵育，作为细胞复制和基质合成的指标。生长调节素活性或软骨同位素摄取的个体值在一窝内进行排序。在每种情况下，注射胰岛素的胎儿在窝内的排名（而非注射生理盐水的胎儿）显著高于该窝的平均排名。胰岛素在体外不刺激软骨代谢。
