Killian C S, Vargas F P, Lee C L, Wang M C, Murphy G P, Chu T M
Invest Urol. 1980 Nov;18(3):219-24.
We developed two quantitative counterimmunoelectrophoretic (CIEP) assays to detect serum prostatic acid phosphatase (PAP). The assays were designated counterimmunoelectrophoresis-colorimetric (CIE-C) and counterimmunoelectrophoresis-densitometric (CIE-D). One unique feature of these assays was the use of a primary standard for the quantitation of PAP. The sensitivities of the assays were 0.08 and 0.04 IU per liter for CIE-C and CIE-D, respectively. Precision coefficients of variation and recovery studies for four levels of PAP over a 3-month period have shown the reliability of these newly modified assays. PAP activity in sera from patients with early stages of prostatic cancer which was demonstrated to be normal by conventional chemical methods was found to be elevated by the conbined methods in a substantial number.
我们开发了两种定量反向免疫电泳（CIEP）检测方法来检测血清前列腺酸性磷酸酶（PAP）。这两种检测方法分别称为反向免疫电泳-比色法（CIE-C）和反向免疫电泳-光密度法（CIE-D）。这些检测方法的一个独特之处是使用了用于定量PAP的一级标准品。CIE-C和CIE-D检测方法的灵敏度分别为每升0.08和0.04国际单位。在3个月的时间里，对四个PAP水平进行的精密度变异系数和回收率研究表明了这些新改良检测方法的可靠性。通过联合方法发现，许多经传统化学方法证明正常的前列腺癌早期患者血清中的PAP活性升高。
