Schaak J, Mao J, Söll D
Nucleic Acids Res. 1982 May 11;10(9):2851-64. doi: 10.1093/nar/10.9.2851.
We have characterized the rRNA gene repeat in Schizosaccharomyces pombe. This repeat, which does not contain the 5S RNA gene, is found in a 10.4 kb HindIII DNA fragment. We have determined the nucleotide sequences of the S. pombe 5.8S RNA gene and intergenic spacers from two different 10.4 kb DNA fragments. Analysis of isolated total cellular 5.8S RNA revealed the presence of eight species of 5.8S RNA, differing in the number of nucleotides at the 5'-end. The eight 4.8S RNA species vary in length from 158 to 165 nucleotides. Apart from the heterogeneity observed at the 5'-end, the sequence of the eight 5.8S RNA species appears to be identical and is the same sequence as coded for by the 5.8S genes. The gene sequence shows great homology to the 5.8S RNA genes or S. cerevisiae and N. crassa. Most of the base differences are confined to the highly variable stem though to be involved in co-axial helix stacking with the 25S RNA, where base pairing is nearly identical despite the sequence differences. Secondary structure models are examined in light of 5.8S RNA oligonucleotide conservation across species from yeasts to higher eukaryotes.
我们已经对粟酒裂殖酵母中的rRNA基因重复序列进行了特征分析。这个不包含5S RNA基因的重复序列存在于一个10.4 kb的HindIII DNA片段中。我们测定了来自两个不同的10.4 kb DNA片段的粟酒裂殖酵母5.8S RNA基因和基因间隔区的核苷酸序列。对分离得到的总细胞5.8S RNA的分析显示存在8种5.8S RNA，它们在5'端的核苷酸数量上有所不同。这8种4.8S RNA的长度在158到165个核苷酸之间变化。除了在5'端观察到的异质性外，这8种5.8S RNA的序列似乎是相同的，并且与5.8S基因编码的序列相同。该基因序列与酿酒酵母和粗糙脉孢菌的5.8S RNA基因具有高度同源性。大多数碱基差异局限于高度可变的茎区，尽管该茎区参与与25S RNA的共轴螺旋堆积，尽管序列不同，但碱基配对几乎相同。根据从酵母到高等真核生物的不同物种间5.8S RNA寡核苷酸的保守性，对二级结构模型进行了研究。
