粪生球壳菌中异常紧密的核糖体RNA基因簇。
Unusually compact ribosomal RNA gene cluster in Sphaeronaemella fimicola.
作者信息
Hausner G, Klassen G R, Reid J
机构信息
Department of Botany, University of Manitoba, Winnipeg, Canada.
出版信息
Curr Genet. 1993;23(4):357-9. doi: 10.1007/BF00310899.
The ribosomal DNA repeat unit of Sphaeronaemella fimicola was found to be a 13.7-kb tandem repeat with a relatively long nontranscribed spacer (NTS) and an unusually compact ribosomal RNA gene cluster. The DNA sequence of an 850-bp PCR amplification product containing the 3' end of the small subunit rRNA (SSrRNA) gene, the 5.8s gene, and the 5' end of the large subunit rRNA (LSrRNA) gene was determined. The putative internal spacers flanking the 5.8s RNA gene could be the shortest yet noted for any fungus, totaling only 102 bases.
发现粪生球腔菌的核糖体DNA重复单元是一个13.7千碱基对的串联重复序列，具有相对较长的非转录间隔区（NTS）和异常紧凑的核糖体RNA基因簇。测定了一个850碱基对PCR扩增产物的DNA序列，该产物包含小亚基rRNA（SSrRNA）基因的3'端、5.8s基因和大亚基rRNA（LSrRNA）基因的5'端。5.8s RNA基因两侧的推定内部间隔区可能是迄今所发现的任何真菌中最短的，总共只有102个碱基。
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