Klevan L, Dattagupta N, Hogan M, Crothers D M
Biochemistry. 1978 Oct 17;17(21):4533-40. doi: 10.1021/bi00614a027.
Biochemical, spectroscopic, and hydrodynamic studies were performed on the reconstituted complex of 140 base pair DNA and the arginine-rich histone tetramer (H3/H4)2. The histones bind to DNA in a 1:1 molar ration to form a stable particle which orients in an electric field with a rotational correlation time of 6.3 mus and a limitign reduced dichroism of --0.74. The complex was modeled hydrodynamically as a cylinder of dimensions 450 X 80 X 80 A containing approximately 1.5 superhelical turns. Addition of the lysinerich histones to this complex cause a condensation of the structure and results in physical properties nearly identical with those of a native nucleosomal particle.
对由140个碱基对的DNA和富含精氨酸的组蛋白四聚体（H3/H4）2重构而成的复合物进行了生化、光谱和流体动力学研究。组蛋白以1:1的摩尔比与DNA结合，形成一个稳定的颗粒，该颗粒在电场中定向，旋转相关时间为6.3微秒，极限减色为-0.74。该复合物在流体动力学上被模拟为一个尺寸为450×80×80埃的圆柱体，包含约1.5个超螺旋圈。向该复合物中添加富含赖氨酸的组蛋白会导致结构凝聚，并产生与天然核小体颗粒几乎相同的物理性质。
