体外翻译过程中蛋白质合成内部起始位点的激活。

Nucleic Acids Res. 1980 Mar 25;8(6):1307-17. doi: 10.1093/nar/8.6.1307.

The major mRNA for adenovirus 2 polypeptide pVIII sediments at 18S as assayed by in vitro translation in the messenger-dependent rabbit reticulocyte lysate system. However, a small amount of messenger activity for pVIII sediments at abut 27S, coincident with the mRNA for 100 K. Isolation and fractionation of poly(A) containing RNA following in vitro translation of 27S 100 K mRNA demonstrated the appearance of an 18S messenger activity for pVIII, which is approximately the size of the authentic mRNA for this protein. Partial degradation of 27S 100 K mRNA with alkali or ribonuclease T1 also results in activation of an 18S messenger activity for pVIII suggesting that in vitro messenger activity for pVIII associated with 27S RNA is due to degradation of 100 K mRNA during translation in the cell-free system.

在信使依赖的兔网织红细胞裂解物系统中通过体外翻译测定，腺病毒2多肽pVIII的主要mRNA沉降系数为18S。然而，少量pVIII的信使活性沉降系数约为27S，与100K的mRNA一致。对27S 100K mRNA进行体外翻译后，对含poly(A)的RNA进行分离和分级分离，结果显示出现了pVIII的18S信使活性，其大小与该蛋白的真实mRNA大小相近。用碱或核糖核酸酶T1对27S 100K mRNA进行部分降解，也会导致pVIII的18S信使活性被激活，这表明与27S RNA相关的pVIII体外信使活性是由于在无细胞系统翻译过程中100K mRNA的降解所致。