Gerlinger P, Mohier E, Le Meur M A, Hirth L
Nucleic Acids Res. 1977 Apr;4(4):813-26. doi: 10.1093/nar/4.4.813.
The four alfalfa mosaic virus RNAs (respectively 24 S, 20 S, 17 S and 12 S) have been used separately as messengers in two in vitro protein synthesizing systems: wheat germ and rabbit reticulocyte lysate. In both systems a polypeptide corresponding to the translation of the entire length of the RNA can be found for RNAs 24 S, 20 S and 12 S, but not for 17 S RNA, the translation product of which is only 35,000 daltons. The number of initiation sites has been determined for each RNA by analyzing the initiation peptides synthesized in the presence of spasomycin and show that there is only one initiation or binding site perRNA. We thus conclude that each AMV RNA behaves as a monocistronic messenger in in vitro translating systems.
苜蓿花叶病毒的四种RNA（分别为24S、20S、17S和12S）已分别在两种体外蛋白质合成系统中用作信使：小麦胚芽和兔网织红细胞裂解物。在这两种系统中，对于24S、20S和12S RNA，都能找到与RNA全长翻译相对应的多肽，但对于17S RNA则找不到，其翻译产物仅为35,000道尔顿。通过分析在斯巴霉素存在下合成的起始肽，确定了每种RNA的起始位点数量，结果表明每个RNA只有一个起始或结合位点。因此，我们得出结论，在体外翻译系统中，每种苜蓿花叶病毒RNA都表现为单顺反子信使。
