Ko L, Koestner A, Wechsler W
Acta Neuropathol. 1980;51(2):107-11. doi: 10.1007/BF00690451.
Aberrant biological characteristics of established cell lines and clones, derived from nitrosourea-induced gliomas in CDF rats were compared with normal rat glial cells. Despite their tumorigenicity and unrestrained proliferation due to inherent cytogenetic anomalies, these neoplastic cells retained certain normal glial attributes to a variable degree. Differentiated glioma cells resembled normal glial cells to a greater extent than they resembled anaplastic glioma cells. They were also less malignant upon implantation. Cell cycle analyses revealed that considerable variations not only for the G1 phase but also for the S period were demonstrated among different tumor cell types. Shortening of the G1 phase may dictate a shorter generation time (shorter doubling time) since a larger potential proliferative pool may overcome the effect of a prolonged generation period may constitute a faster proliferation rate. Although the cell generation period of neoplastic cells is not necessarily shorter than that of normal glial cells, the lower proportion of nonproliferative cells results in a much faster growth rate when compared wo non-neoplastic glial cells in culture.
将源自CDF大鼠亚硝基脲诱导的胶质瘤的已建立细胞系和克隆的异常生物学特性与正常大鼠神经胶质细胞进行了比较。尽管这些肿瘤细胞由于固有的细胞遗传学异常而具有致瘤性和不受控制的增殖能力，但它们在不同程度上仍保留了某些正常神经胶质细胞的属性。分化的胶质瘤细胞与正常神经胶质细胞的相似程度大于与间变性胶质瘤细胞的相似程度。它们在植入时的恶性程度也较低。细胞周期分析表明，不同肿瘤细胞类型不仅在G1期，而且在S期都表现出相当大的差异。G1期的缩短可能意味着世代时间（加倍时间）更短，因为更大的潜在增殖池可能克服延长世代期的影响，这可能构成更快的增殖速率。虽然肿瘤细胞的细胞世代期不一定比正常神经胶质细胞短，但与培养中的非肿瘤性神经胶质细胞相比，非增殖细胞比例较低导致生长速度快得多。
