Craig P S, Mitchell G F, Cruise K M, Rickard M D
Aust J Exp Biol Med Sci. 1980 Aug;58(4):339-50. doi: 10.1038/icb.1980.33.
Attempts have been made to produce discriminatory immunodiagnostic reagents for infections of sheep with the larval stages of Taenia hydatigena, Taenia ovis and Echinococcus granulosus. Using cells from mice hyperimmunized with T. hydatigena antigenic preparations for fusion, a hybridoma antibody which bound to T. hydatigena larval antigens, but not to T. ovis or E. granulosus larval antigens, was selected. A competitive radioimmunoassay using the labelled hybridoma antibody and a crude parasite antigen mixture was devised as an immunodiagnostic test for experimental T. hydatigena infection. Sera from sheep exposed to 1000 or more T. hydatigena eggs were unequivocally positive but sera from 2 sheep with a monospecific infection with T. ovis were also positive. Sera from E. granulosus-infected sheep were negative and, although the numbers of sera involved were small, such discrimination has not been achieved by previous serologic methods. The discussion emphasizes various difficulties which have been encountered in the development of highly sensitive as well as specific hybridoma-based immunodiagnostic reagents for the detection of larval cestode infections in sheep.
人们已尝试制备用于诊断绵羊感染泡状带绦虫、绵羊绦虫和细粒棘球绦虫幼虫阶段的鉴别性免疫诊断试剂。利用用泡状带绦虫抗原制剂超免疫的小鼠细胞进行融合，筛选出一种杂交瘤抗体，它能与泡状带绦虫幼虫抗原结合，但不与绵羊绦虫或细粒棘球绦虫幼虫抗原结合。设计了一种使用标记的杂交瘤抗体和粗制寄生虫抗原混合物的竞争性放射免疫测定法，作为实验性泡状带绦虫感染的免疫诊断试验。暴露于1000个或更多泡状带绦虫卵的绵羊血清呈明确阳性，但2只单特异性感染绵羊绦虫的绵羊血清也呈阳性。细粒棘球绦虫感染绵羊的血清呈阴性，尽管所涉及的血清数量较少，但以前的血清学方法尚未实现这种鉴别。讨论强调了在开发用于检测绵羊绦虫幼虫感染的高灵敏度和特异性基于杂交瘤的免疫诊断试剂过程中遇到的各种困难。
