大鼠肾脏和肝脏中结合重金属的低分子量蛋白质的免疫学特性

Immunological properties of low molecular-weight proteins binding heavy metals in rat kidney and liver.

作者信息

Zelazowski A J, Szymańska J A, Cierniewski C S

出版信息

Chem Biol Interact. 1980 Dec;33(1):115-25. doi: 10.1016/0009-2797(80)90048-4.

Abstract

Two homogenous fractions of hepatic metallothioneins ((Cd,Zn) MT-1 and (Cd,Zn) MT-2) and renal metal binding proteins ((Bi,Cu) BP-1 and (Bi,Cu) BP-2) were isolated from rats exposed to heavy metals and specific antisera to them were produced in rabbits. These antisera were tested by immunodiffusion and immunoelectrophoresis for their ability to bind different fractions of hepatic Cd,Zn-metallothionein and renal (Bi,Cu)-, (Hg,Cu)- and (Cd,Cu)-binding proteins. It was found that anti (Bi,Cu) BP antisera did not cross-react with hepatic (Cd,Zn) MT-1 and (Cd,Zn) MT-2. Strong immunological cross-reactions were detected between anti (Bi,Cu) BP antisera and individual forms of (Cd,Cu)-, (Hg,Cu)- and (Bi,Cu)-binding proteins isolated from rat kidneys.

摘要

从暴露于重金属的大鼠中分离出两种肝金属硫蛋白（（镉，锌）MT - 1和（镉，锌）MT - 2）和肾金属结合蛋白（（铋，铜）BP - 1和（铋，铜）BP - 2）的均一馏分，并在兔体内制备针对它们的特异性抗血清。通过免疫扩散和免疫电泳测试这些抗血清结合肝镉，锌 - 金属硫蛋白和肾（铋，铜） - 、（汞，铜） - 和（镉，铜） - 结合蛋白不同馏分的能力。发现抗（铋，铜）BP抗血清与肝（镉，锌）MT - 1和（镉，锌）MT - 2不发生交叉反应。在抗（铋，铜）BP抗血清与从大鼠肾脏分离出的（镉，铜） - 、（汞，铜） - 和（铋，铜） - 结合蛋白的各个形式之间检测到强烈的免疫交叉反应。