Inoue M, Okubo T, Oshima H, Saito T, Kato M, Mitsuhashi S
J Bacteriol. 1980 Dec;144(3):1186-9. doi: 10.1128/jb.144.3.1186-1189.1980.
Four lysozyme-sensitive mutants were isolated after nitrosoguanidine mutagenesis of a lysozyme-insensitive strain of Staphylococcus aureus. One mutant was sufficiently effective for the isolation of macromolecules, such as plasmid deoxyribonucleic acids, from a cell after lysozyme-induced cell lysis.
在用亚硝基胍对一株对溶菌酶不敏感的金黄色葡萄球菌进行诱变后,分离出了四个对溶菌酶敏感的突变体。其中一个突变体在溶菌酶诱导细胞裂解后,对于从细胞中分离大分子物质(如质粒脱氧核糖核酸)非常有效。