Laidlaw S A, Moldave K
J Biol Chem. 1980 Dec 25;255(24):11908-13.
The effects of beta-endorphin and various enkephalins on protein synthesis in eukaryotic cell-free systems have been examined. Beta-Endorphin, Leu-enkephalin, and Met-enkephalin inhibit the incorporation of radioactive leucine into globin in the presence of reticulocyte poly(A)+ RNA and of radioactive phenylalanine into polyphenylalanine in the presence of poly(U); however, the poly(U)-dependent synthesis of polyphenylalanine from Phe-tRNA is not inhibited. the aminoacylation of tRNAPhe is markedly inhibited by enkephalin, indicating that the sensitive component is Phe-tRNA synthetase. Other aminoacyl-tRNA synthetases are not significantly affected. The interaction between enkephalin and Phe-tRNA synthetase is reversible; activity is restored by dialysis of enzyme-enkephalin reaction mixtures. Morphine, vasopressin and analogues of vasopressin, and enkephalin analogues such as [D-Ala2,D-Leu5]enkephalin and [D-Ala2,Met]enkephalinamide have no effect on translation. The results suggest that the effects on protein synthesis are probably not related to opiate effects.
已经研究了β-内啡肽和各种脑啡肽对真核无细胞系统中蛋白质合成的影响。β-内啡肽、亮氨酸脑啡肽和甲硫氨酸脑啡肽在存在网织红细胞多聚腺苷酸（poly(A)+）RNA的情况下，抑制放射性亮氨酸掺入珠蛋白，在存在聚尿苷酸（poly(U)）的情况下，抑制放射性苯丙氨酸掺入多聚苯丙氨酸；然而，由苯丙氨酰-tRNA合成多聚苯丙氨酸的聚尿苷酸依赖性合成并未受到抑制。脑啡肽显著抑制苯丙氨酰-tRNA（tRNAPhe）的氨酰化，表明敏感成分是苯丙氨酰-tRNA合成酶。其他氨酰-tRNA合成酶未受到明显影响。脑啡肽与苯丙氨酰-tRNA合成酶之间的相互作用是可逆的；通过对酶-脑啡肽反应混合物进行透析可恢复活性。吗啡、血管加压素及其类似物，以及脑啡肽类似物，如[D-Ala2,D-Leu5]脑啡肽和[D-Ala2,Met]脑啡肽酰胺对翻译没有影响。结果表明，对蛋白质合成的影响可能与阿片类药物的作用无关。
