在苯丙氨酸限制条件下哺乳动物苯丙氨酰 - tRNA合成酶的过表达。

Overexpression of mammalian phenylalanyl-tRNA synthetase upon phenylalanine restriction.

作者信息

Lazard M, Mirande M, Waller J P

出版信息

FEBS Lett. 1987 May 25;216(1):27-30. doi: 10.1016/0014-5793(87)80750-0.

Abstract

The effect of phenylalanine restriction on the level of expression of phenylalanyl-tRNA synthetase from cultured Chinese hamster ovary cells was investigated. By lowering the phenylalanine concentration from 200 to 2 microM, cell growth was arrested, tRNAPhe aminoacylation level was rapidly and specifically decreased and phenylalanyl-tRNA synthetase was derepressed. The progressive 2-fold elevation of phenylalanyl-tRNA synthetase level was determined by activity measurement and immunotitration. None of the other aminoacyl-tRNA synthetases tested were significantly affected.

摘要

研究了苯丙氨酸限制对培养的中国仓鼠卵巢细胞中苯丙氨酰 - tRNA合成酶表达水平的影响。通过将苯丙氨酸浓度从200微摩尔降至2微摩尔，细胞生长停滞，tRNAPhe氨酰化水平迅速且特异性降低，苯丙氨酰 - tRNA合成酶被去抑制。通过活性测定和免疫滴定确定苯丙氨酰 - tRNA合成酶水平逐渐升高了2倍。所测试的其他氨酰 - tRNA合成酶均未受到显著影响。