Nakao K, Ochi H, Kawashima M, Tomino S, Sugano H, Matsumoto K
J Natl Cancer Inst. 1978 Feb;60(2):289-90. doi: 10.1093/jnci/60.2.289.
Concentrations of estrogen receptor (ER) and alpha-fetoprotein were determined by dextran-coated charcoal assay and analyzed with Scatchard plots and radioimmunoassay, respectively, in cytosols of 72 human breast cancers. The values for ER ranged from 0 to 340 fmoles/mg cytosol protein. The concentrations of alpha-fetoprotein, which were low in all tumor cytosols examined, ranged from less than 0.1 to 1.1 ng/mg cytosol protein. No positive relationship was found between ER and alpha-fetoprotein concentrations. These results show that ER, but not alpha-fetoprotein, usually accounts for most of the high estrogen-binding capacity in cytosols of human breast cancers.
采用葡聚糖包被活性炭分析法测定了72例人类乳腺癌细胞溶质中雌激素受体（ER）的浓度，并分别用Scatchard图和放射免疫分析法进行分析。ER的值范围为0至340飞摩尔/毫克细胞溶质蛋白。在所检测的所有肿瘤细胞溶质中，甲胎蛋白浓度较低，范围为低于0.1至1.1纳克/毫克细胞溶质蛋白。未发现ER与甲胎蛋白浓度之间存在正相关关系。这些结果表明，在人类乳腺癌细胞溶质中，通常是ER而非甲胎蛋白占高雌激素结合能力的大部分。
