Fournie G J, Minh M G, Mignon-Conte M A, Hass S, Lambert P H, Conte J J
J Clin Lab Immunol. 1980 Sep;4(2):103-6.
The effects of early immunization with DNA and of injection of bacterial lipopolysaccharide (LPS) on the glomerulonephritis of NZB x NZW mice were studied. Combined injections of DNA complexed to methylated bovine serum albumin (DNA-mBSA) and of LPS appeared to be more efficient in accelerating the disease in NZB x NZW mice than injections of DNA-mBSA or LPS alone. A rapid increase in levels of anti-DNA antibodies, an early appearance of severe renal lesions and a shortened survival were observed in mice injected with both DNA-mBSA and LPS. This new model was found to be suitable for therapeutic studies in mice with accelerated disease treated with cyclophosphamide and heparin. The efficacy of cyclophosphamide for the treatment of NZB x NZW mouse disease was shown by immunological and histological studies in mice younger than 4 months. Heparin appeared to have a beneficial effect by preventing the endocapillary cellular proliferation induced by injections of DNA-mBSA and LPS. The accelerated model of NZB x NZW mouse disease might be a useful tool for experiments on the treatment of lupus nephritis.
研究了DNA早期免疫及注射细菌脂多糖（LPS）对NZB×NZW小鼠肾小球肾炎的影响。与甲基化牛血清白蛋白复合的DNA（DNA-mBSA）和LPS联合注射，在加速NZB×NZW小鼠疾病进展方面似乎比单独注射DNA-mBSA或LPS更有效。在同时注射DNA-mBSA和LPS的小鼠中，观察到抗DNA抗体水平迅速升高、严重肾损伤早期出现以及生存期缩短。发现这种新模型适用于用环磷酰胺和肝素治疗的疾病加速小鼠的治疗研究。4个月以下小鼠的免疫学和组织学研究表明了环磷酰胺治疗NZB×NZW小鼠疾病的疗效。肝素似乎具有有益作用，可防止因注射DNA-mBSA和LPS诱导的毛细血管内细胞增殖。NZB×NZW小鼠疾病的加速模型可能是狼疮性肾炎治疗实验的有用工具。
