Auer I O, Ziemer E
Klin Wochenschr. 1980 Aug 1;58(15):779-87. doi: 10.1007/BF01478286.
In Crohn's disease (CD) a potential etiopathogenetic role of the antibody dependent cell mediated cytotoxicity (ADCC) has been discussed. By means of a 51Cr release assay with an established human lymphoblastoid cell line as target, the in vitro ADCC activity was studied in the peripheral blood of 34 patients with CD. 11 patients with inflammatory bowel disease other than CD or ulcerative colitis (group D) and 45 sex and age matched healthy people served as controls. In unseparated mononuclear cell suspensions, we found a significantly lower ADCC activity in CD patients and in group D than in normals. Depletion of both phagocytic and plastic adherent cells, i.e. macrophages, resulted in complete normalisation of the ADCC activity in all patients of group D and also two-thirds of the CD patients. However, the remaining one third CD patients still exhibited ADCC activity below 1 standard deviation of normal. Most of these latter CD patients had highly active disease. Thus, this study showes that the general ADCC per se is normal in CD, except for a decrease in patients with high activity of disease. However, the normal general ADCC activity per se is significantly suppressed by phagocytic, plastic adherent cells. In this regard similar results were found in group D.
在克罗恩病（CD）中，抗体依赖性细胞介导的细胞毒性（ADCC）的潜在病因学作用已被讨论。通过以已建立的人淋巴母细胞系为靶标的51Cr释放试验，研究了34例CD患者外周血中的体外ADCC活性。11例患有除CD或溃疡性结肠炎以外的炎症性肠病的患者（D组）以及45名年龄和性别匹配的健康人作为对照。在未分离的单核细胞悬液中，我们发现CD患者和D组的ADCC活性明显低于正常人。吞噬细胞和塑料贴壁细胞（即巨噬细胞）的清除导致D组所有患者以及三分之二的CD患者的ADCC活性完全恢复正常。然而，其余三分之一的CD患者的ADCC活性仍低于正常标准差1倍。这些后期CD患者大多数患有高度活动性疾病。因此，本研究表明，除了疾病活动度高的患者有所降低外，CD患者的总体ADCC本身是正常的。然而，吞噬性塑料贴壁细胞会显著抑制正常的总体ADCC活性。在这方面，D组也发现了类似的结果。
