胶原蛋白DNA的R环杂交：在硫酸铯梯度中的分离

R-loop hybridization of collagen DNA: separation in Cs2SO4 gradients.

作者信息

Born J, Wittig B, Wittig S, Tiedemann H

出版信息

Mol Biol Rep. 1980 Oct 31;6(3):159-61. doi: 10.1007/BF00775410.

Abstract

High molecular weight poly(A)RNA (26-35 S) from chicken embryo trunks which is enriched for collagen mRNA was iodinated and hybridized to DNA under conditions of R-loop formation. The R-loops were separated in Cs2SO4 gradients from the bulk of DNA yielding double stranded DNA enriched for collagen genes.

摘要

从鸡胚躯干中提取的富含胶原蛋白mRNA的高分子量多聚腺苷酸RNA（26 - 35S）经过碘化处理，并在R环形成条件下与DNA杂交。R环在Cs2SO4梯度中与大部分DNA分离，从而得到富含胶原蛋白基因的双链DNA。