Cortadas J, Olofsson B, Meunier-Rotival M, Macaya G, Bernardi G
Eur J Biochem. 1979 Aug 15;99(1):179-86. doi: 10.1111/j.1432-1033.1979.tb13244.x.
The organization of the chicken genome was investigated by centrifuging chicken DNA (Mr = 57 X 10(6) in preparative Cs2SO4/Ag+ and Cs2SO4/BAMD density gradients [BAMD = 3.6-bis(acetato-mercurimethyl)dioxane]. An analysis by CsCl density gradient of the DNA fractions obtained from the preparative experiments revealed that 88% of the genome is made up of four DNA components, characterized by buoyant densities of 1.699, 1.702(5), 1.704(5) and 1.708 g/cm3 and representing 39%, 25%, 15%, and 9%, respectively, of the total DNA. The remaining 12% of the genome is formed by seven minor and/or satellite components. The distribution of the ovalbumin gene in a Cs2CO4/BAMD density gradient, as tested with a cloned cDNA probe, coincides with the distribution of the 1.702(5)-g/cm3 component. This shows that the DNA regions flanking the ovalbumin gene are homogeneous in base composition over along distances and that the gene is located on a DNA segment belonging to the 1.702(5)-g/cm3 component.
通过在制备性Cs2SO4/Ag+和Cs2SO4/BAMD密度梯度[BAMD = 3.6 - 双（乙酰氧基 - 汞甲基）二氧六环]中对鸡DNA（Mr = 57×10(6)）进行离心，研究了鸡基因组的组织情况。对从制备性实验中获得的DNA组分进行CsCl密度梯度分析表明，88%的基因组由四种DNA成分组成，其特征性浮力密度分别为1.699、1.702(5)、1.704(5)和1.708 g/cm3，分别占总DNA的39%、25%、15%和9%。基因组的其余12%由七种次要和/或卫星成分组成。用克隆的cDNA探针测试，卵清蛋白基因在Cs2CO4/BAMD密度梯度中的分布与1.702(5)-g/cm3成分的分布一致。这表明卵清蛋白基因侧翼的DNA区域在很长距离内碱基组成是均匀的，并且该基因位于属于1.702(5)-g/cm3成分的DNA片段上。
