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G带小鼠胚胎染色体侧向不对称性的显示

Demonstration of lateral asymmetry in G-banded mouse embryo chromosomes.

作者信息

Tucci S M, Skalko R G

出版信息

Experientia. 1977 Nov 15;33(11):1437-8. doi: 10.1007/BF01918791.

Abstract

Cells obtained from 10-day mouse embryos were cultured for 3 h in medium containing colcemid. Chromosome preparations were subjected to G-banding in either calcium-magnesium free Hank's solution or the ASG method. From one to several occurrences of lateral asymmetry (unequal banding of sister chromatids) were observed in the majority of karytotypes analyzed.

摘要

从10日龄小鼠胚胎获取的细胞在含有秋水仙酰胺的培养基中培养3小时。染色体标本制备采用无钙镁的汉克氏溶液或ASG方法进行G显带。在所分析的大多数核型中观察到一至数次侧向不对称现象(姐妹染色单体带型不等)。

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