Beach D H, Mueller J F, Holz G G
Mol Biochem Parasitol. 1980 Sep;1(5):249-68. doi: 10.1016/0166-6851(80)90059-6.
The kinds and amounts of the lipids of each stage (egg, coracidium, procercoid, plerocercoid, adult) in the life-cycle of the cestode Spirometra mansonoides, and of environmental lipids, have been examined by combinations of TEAE-cellulose and silicic acid column, silica gel thin-layer and SCOT column gas-liquid chromatography. Major lipids of all stages were triacylglycerols, cholesterol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, and phosphatidylcholine. Minor lipids were sterol esters, fatty acids, benzoquinones, partial glycerides phosphatidic acid, sphingolipids and lysolipids. Triacylglycerols decreased and cholesterol and phospholipids, particularly diphosphatidylglycerol and phosphatidylcholine, increased during embryogenesis (egg to coracidium). Neutral and phosphoglycerides had characteristic fatty acyl group patterns irrespective of life-cycle stage, except for procercoid lipids, which were unique in their content of branched and odd-numbered forms. The patterns seen in the total lipids of the various life-cycle stages were qualitatively similar to those of the environments of those stages, but were often quantitatively dissimilar.
用三乙胺纤维素和硅酸柱、硅胶薄层及SCOT柱气液色谱法相结合，对曼氏迭宫绦虫生命周期中各阶段（虫卵、钩球蚴、原尾蚴、裂头蚴、成虫）的脂质种类和含量以及环境脂质进行了检测。所有阶段的主要脂质为三酰甘油、胆固醇、二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰丝氨酸、磷脂酰肌醇和磷脂酰胆碱。次要脂质为甾醇酯、脂肪酸、苯醌、甘油二酯、磷脂酸、鞘脂和溶血脂质。在胚胎发育过程中（从虫卵到钩球蚴），三酰甘油减少，而胆固醇和磷脂，尤其是二磷脂酰甘油和磷脂酰胆碱增加。中性和磷酸甘油酯具有特征性的脂肪酰基模式，与生命周期阶段无关，但原尾蚴脂质除外，其独特之处在于含有支链和奇数形式的脂质。不同生命周期阶段总脂质中的模式在质量上与这些阶段的环境相似，但在数量上往往不同。
