5型腺病毒DNA转化性HpaI - E片段的核苷酸序列。

The nucleotide sequence of the transforming HpaI-E fragment of adenovirus type 5 DNA.

作者信息

Van Ormondt H, Maat J, De Waard A, Van der Eb A J

出版信息

Gene. 1978 Dec;4(4):309-28. doi: 10.1016/0378-1119(78)90048-3.

Abstract

The primary structure of the HpaI-E fragment of adenovirus type 5 (Ad5) DNA has been determined, mainly by the method of Maxam and Gilbert (1977). This fragment comprises the leftmost 4.5% of the Ad5 genome, and has been shown to be the shortest DNA fragment capable of transforming cells. The identification of potential initiation and termination codons in the determined sequence indicates that two small polypeptides consisting of 186, and 81 amino acids, respectively, could be synthesized. Taking into account recent data on RNA splicing, a possibility is considered that this DNA may code also for larger polypeptides.

摘要

已主要通过马克萨姆和吉尔伯特（1977年）的方法确定了5型腺病毒（Ad5）DNA的HpaI-E片段的一级结构。该片段包含Ad5基因组最左边的4.5%，并且已被证明是能够转化细胞的最短DNA片段。在所确定序列中潜在起始密码子和终止密码子的鉴定表明，可能分别合成由186个和81个氨基酸组成的两种小多肽。考虑到最近关于RNA剪接的数据，认为这种DNA也可能编码更大的多肽。