Valdes R, Savory G, Bruns D, Renoe B, Savory J, Wills M R
Clin Chem. 1979 Jul;25(7):1254-8.
We report an evaluation of the GammafloTM automated continuous-flow radioimmunoassay instrument in which we used a digoxin assay to assess system performance. System operation was based on combined continuous-flow and column-chromatographic techniques. No drift or carryover was detectable in 180 within-assay consecutive determinations performed at a rate of 42 determinations per hour (5 h of continuous operation). Within-assay and between-assay precision were less than 6% (coefficient of variation). The automated method correlated well (r = 0.960 and 0.952, respectively) with two established manual digoxin radioimmunoassay procedures. The data suggest this automated system offers a valid alternative to manual radioimmunoassay procedures in terms of overall precision, simplicity of operation, and sample throughout capacity.
我们报告了对GammafloTM自动连续流动放射免疫分析仪器的评估，其中我们使用地高辛分析来评估系统性能。系统操作基于连续流动和柱色谱技术的结合。以每小时42次测定的速率（连续操作5小时）进行的180次批内连续测定中未检测到漂移或残留。批内和批间精密度均小于6%（变异系数）。该自动化方法与两种既定的手动地高辛放射免疫分析方法具有良好的相关性（分别为r = 0.960和0.952）。数据表明，就整体精密度、操作简便性和样品通量而言，该自动化系统为手动放射免疫分析方法提供了有效的替代方案。
