Lerner R A, Hodge L D
Proc Natl Acad Sci U S A. 1969 Oct;64(2):544-51. doi: 10.1073/pnas.64.2.544.
The RNA synthesis of a single-stranded, multisegmented viral genome has been investigated in nonpermissive host cells. In HeLa cells, both single- and double-stranded influenza viral RNA were made and accumulated, and synthesis was completed by 15 hours. The evidence indicated that all pieces of the genome were synthesized. At least as much viral RNA was synthesized in HeLa cells as in permissive chick embryo fibroblasts cells. Explanations for this nonpermissive infection based on reduced synthesis of viral RNA or rapid destruction of newly synthesized RNA, uncontrolled synthesis of RNA, or partial synthesis of the genome appear to have been ruled out by this investigation. The persistently high amounts of double-stranded RNA, combined with the reported lack of dispersion of nucleocapsid protein from the nuclear region of the HeLa cell are consistent with a failure of assembly of the virion in this host. This nonpermissive infection of HeLa cells with influenza virus may offer a unique system for the accumulation of the intermediates involved in the assembly of a complex RNA viral genome.
在非允许性宿主细胞中研究了单链、多节段病毒基因组的RNA合成。在HeLa细胞中，单链和双链流感病毒RNA均能产生并积累，且合成在15小时内完成。证据表明基因组的所有片段均已合成。HeLa细胞中合成的病毒RNA至少与允许性鸡胚成纤维细胞中的一样多。基于病毒RNA合成减少或新合成RNA的快速破坏、RNA的无控制合成或基因组的部分合成来解释这种非允许性感染，似乎已被该研究所排除。持续大量的双链RNA，再加上报道的核衣壳蛋白在HeLa细胞核区域缺乏分散，这与该宿主中病毒粒子组装失败是一致的。HeLa细胞被流感病毒的这种非允许性感染可能为积累参与复杂RNA病毒基因组组装的中间体提供一个独特的系统。
