噬菌体分型、血清分型和生物分型在克雷伯菌感染流行病学监测中的辅助作用比较。
Comparison of bacteriophage typing, serotyping, and biotyping as aids in epidemiological surveillance of Klebsiella infections.
作者信息
Rennie R P, Nord C E, Sjoberg L, Duncan I B
出版信息
J Clin Microbiol. 1978 Dec;8(6):638-42. doi: 10.1128/jcm.8.6.638-642.1978.
Abstract
Bacteriophage typing was used to subdivide Klebsiella obtained from patients in a surgical intensive care unit during a 2-year period. The 15 phages employed to type the strains were propagated by a soft-agar layer technique. In all, 23 phage types were found among the 120 clinical strains. The phage types of repeat isolates were reproducible. Only 70% of the strains tested were phage typable, but when used in conjunction with capsular serotyping and biotyping, a much greater subdivision of the Klebsiella strains was achieved. The addition of phage typing to serobiotyping for epidemiological analysis suggested that the number of cross-infecting Klebsiella strains in the intensive care unit was few, but that these strains persisted in the unit for long periods of time and could infect different body sites.
摘要
在两年期间，利用噬菌体分型法对从外科重症监护病房患者身上分离出的克雷伯菌进行细分。用于菌株分型的15种噬菌体通过软琼脂层技术进行增殖。在120株临床菌株中，共发现了23种噬菌体类型。重复分离株的噬菌体类型具有可重复性。仅70%的测试菌株可进行噬菌体分型，但当与荚膜血清分型和生物分型结合使用时，对克雷伯菌菌株的细分程度更高。将噬菌体分型添加到血清生物分型中进行流行病学分析表明，重症监护病房中交叉感染的克雷伯菌菌株数量较少，但这些菌株在病房中持续存在很长时间，并可感染不同身体部位。
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