Demers D M, Fukushima S, Cohen S M
Cancer Res. 1981 Jan;41(1):108-12.
We examined several parameters of urine excretion during a two-year initiation-promotion experiment in male Fischer rats using four weeks of N-[4-(5-nitro-2-furyl)-2-thiazoly]formamide at 0.2% of the diet as the initiating agent and either 5% sodium saccharin or 2% L-tryptophan in the diet as promoting agents. Rats fed sodium saccharin increased their intake of water; this was accompanied by diarrhea and an increased urinary volume. Osmolality was decreased slightly. The total amount of sodium excreted was increased, although the concentration in the urine was similar to that of the controls or slightly increased. No abnormalities were observed in urinary potassium, calcium, urea, or other parameters measured except for the pH, which was slightly increased during the first three months of the experiment. There was no increase in the size or concentration of crystals in the urine of rats fed sodium saccharin, and no calculi were observed. Hypoglycemia and hypoglycosuria were present in sodium saccharin-fed rats and to a lesser extent in L-tryptophan-fed rats. No other abnormalities were seen in the urine of rats fed L-tryptophan. These data suggest that none of the urinary factors measured in our experiment, including crystal and calculus formation, correlated with the induction of urinary bladder lesions by sodium saccharin.
在一项为期两年的启动-促进实验中，我们以0.2%添加于饲料中的N-[4-(5-硝基-2-呋喃基)-2-噻唑基]甲酰胺作为启动剂，以饲料中5%的糖精钠或2%的L-色氨酸作为促进剂，对雄性Fischer大鼠进行了研究，检测了尿液排泄的几个参数。喂食糖精钠的大鼠饮水量增加；同时伴有腹泻和尿量增加。尿渗透压略有降低。钠的排泄总量增加，尽管尿中钠的浓度与对照组相似或略有升高。除了在实验的前三个月pH值略有升高外，在尿钾、钙、尿素或其他检测参数中未观察到异常。喂食糖精钠的大鼠尿液中晶体的大小或浓度没有增加，也未观察到结石。喂食糖精钠的大鼠出现低血糖和糖尿，喂食L-色氨酸的大鼠程度较轻。喂食L-色氨酸的大鼠尿液中未发现其他异常。这些数据表明，在我们的实验中所检测的任何尿液因素，包括晶体和结石的形成，都与糖精钠诱导膀胱病变无关。
