Robertson W S
Clin Chem. 1981 Jan;27(1):144-6.
Some modifications of the conditions of the reaction between plasma and bromcresol green have led to an improved method for determination of plasma albumin with the Vickers M300 multichannel analyzer. Dye concentration and reaction time are the factors principally influencing method specificity, but variable protein composition of human plasma also affects it, so that optimal reaction conditions vary from specimen to specimen. Thus a compromise must be reached such that the best conditions for determining plasma albumin over a range of different protein concentrations are achieved. In the proposed method for the Vickers M300 a reaction time of 12 s (the minimum possible) is used. Comparison with "rocket" immunoelectrophoresis gave the following linear regression: y = 10 + 0.79 x (n = 91; r = 0.96).
对血浆与溴甲酚绿反应条件进行的一些改进，形成了一种利用维氏M300多通道分析仪测定血浆白蛋白的改良方法。染料浓度和反应时间是主要影响方法特异性的因素，但人血浆中可变的蛋白质组成也会对其产生影响，因此最佳反应条件因样本而异。因此，必须达成一种折衷方案，以便在一系列不同蛋白质浓度范围内实现测定血浆白蛋白的最佳条件。在所提出的维氏M300方法中，使用了12秒（可能的最短时间）的反应时间。与“火箭”免疫电泳法比较得出以下线性回归方程：y = 10 + 0.79x（n = 91；r = 0.96）。
