Bültmann B, Geitner R, Seibold H, Kratsch G, Haferkamp O
Klin Wochenschr. 1980 Jul 15;58(14):727-32. doi: 10.1007/BF01478460.
The sera and peripheral blood granulocytes of 10 patients with rheumatoid arthritis and of 10 healthy controls were investigated for the presence of soluble immune complexes and for cellular dysfunction. Using the Rajicell-radioimmunoassay, immune complexes were detected in 7 out of 10 rheumatoid sera. In one patient the presence of immune complexes was demonstrated by immunofluorescence. In all rheumatoid patients with circulating immune complexes decreased chemotactic reactivity and diminished bactericidal capacity of the neutrophils were observed. Incubation of rheumatoid granulocytes with pooled AB-serum or pretreatment of neutrophils of healthy controls with immune complexes containing rheumatoid sera resulted in a reduced number of comparable cellular dysfunctions including increased release of lysosomal enzymes, strongly correlated with the presence of intracellular phagocytosed immune complexes. Phagocytosis and increase of oxidative cell metabolism during phagocytosis were not influenced by circulating immune complexes. Based on our in vitro findings we suggest that the described immune complex-dependent granulocyte dysfunctions are possible responsible for the high risk of local or systemic bacterial diseases in patients with rheumatoid arthritis.
对10例类风湿性关节炎患者和10名健康对照者的血清及外周血粒细胞进行了可溶性免疫复合物检测和细胞功能障碍研究。采用拉氏细胞放射免疫分析法，在10份类风湿血清中有7份检测到免疫复合物。1例患者通过免疫荧光法证实存在免疫复合物。在所有循环免疫复合物阳性的类风湿患者中，均观察到中性粒细胞趋化反应性降低和杀菌能力减弱。将类风湿粒细胞与混合AB血清孵育，或用含类风湿血清的免疫复合物预处理健康对照者的中性粒细胞，导致包括溶酶体酶释放增加在内的类似细胞功能障碍数量减少，这与细胞内吞噬的免疫复合物的存在密切相关。吞噬作用及吞噬过程中细胞氧化代谢的增加不受循环免疫复合物的影响。基于我们的体外研究结果，我们认为所描述的免疫复合物依赖性粒细胞功能障碍可能是类风湿性关节炎患者发生局部或全身细菌感染高风险的原因。
