Breedveld F C, Lafeber G J, de Vries E, Leyh P C, Daha M R, Cats A
Clin Exp Immunol. 1984 Mar;55(3):677-83.
Receptors for the Fc part of IgG on polymorphonuclear cells (FcR) of patients with rheumatoid arthritis (RA), Felty's syndrome (FS) and healthy controls (HC) were studied by means of a rosetting technique with rabbit IgG coated ox erythrocytes. When cold isolated polymorphonuclear cells (PMN) were incubated at room temperature the percentage of rosette forming PMN (RF-PMN) from HC was more than twice that measured directly after isolation at 4 degrees C. The same phenomenon was observed for PMN from patients with RA although the RF-PMN increased by only 1/3. In contrast warming of PMN from patients with FS did not influence the RF-PMN. The presence of surface bound immunoglobulins and intracytoplasmic immunoglobulins was measured by immunofluorescence and appeared to be inversely related to the RF-PMN. A good correlation between the results of the C1q binding assay (C1qBA) and the immunofluorescence score was observed but no correlation existed between the C1qBA and the RF-PMN. These results indicate that the number of PMN expressing FcR in patients with RA and FS is decreased presumably because of the phagocytosis of immune complex like material. The decrease in the availability of FcR may influence the functions of the PMN.
采用兔IgG包被的牛红细胞花环技术，研究了类风湿关节炎（RA）患者、费尔蒂综合征（FS）患者及健康对照者（HC）多形核细胞（PMN）上IgG的Fc段受体（FcR）。当冷分离的多形核细胞（PMN）在室温下孵育时，健康对照者中形成花环的PMN（RF-PMN）百分比是4℃直接分离后所测值的两倍多。类风湿关节炎患者的PMN也观察到同样现象，尽管RF-PMN仅增加了1/3。相比之下，费尔蒂综合征患者的PMN升温对RF-PMN没有影响。通过免疫荧光法检测表面结合免疫球蛋白和胞质内免疫球蛋白的存在情况，结果显示其与RF-PMN呈负相关。观察到C1q结合试验（C1qBA）结果与免疫荧光评分之间有良好相关性，但C1qBA与RF-PMN之间无相关性。这些结果表明，类风湿关节炎和费尔蒂综合征患者中表达FcR的PMN数量减少，可能是由于免疫复合物样物质的吞噬作用。FcR可用性的降低可能会影响PMN的功能。
