阿霉素在体内和体外对肿瘤细胞的细胞毒性。
Cytotoxicity of adriamycin to tumour cells in vivo and in vitro.
作者信息
Martin W M, McNally N J
出版信息
Br J Cancer. 1980 Dec;42(6):881-9. doi: 10.1038/bjc.1980.336.
Two mouse sarcomas have been used to assess the sensitivity to Adriamycin of tumour cells in vivo and in vitro. Both tumours were tissue-culture adapted so that cell survival could be assayed in vitro after treatment either in vivo or in vitro. For both tumours (WHFIB and CBSAF) cells were highly sensitive when treated in vitro yet very resistant to treatment in vivo, whether assayed by cell survival or regrowth delay. Cells from both tumours treated in vitro with Adriamycin immediately after excision were slightly more resistant than the cells maintained in vitro. However, this was not adequate to explain the marked discrepancy between in vivo resistance and in vitro sensitivity. The discrepancy was not due to a failure of drug delivery. Phase of cell growth was the factor was most significantly affecting chemosensitivity in vitro, plateau-phase cells being much more resistant than log-phase cells. Hypoxia was also an important factor leading to reduced chemosensitivity. Tumour diameter, in the range 2-8 mm, did not appear to be important.
两种小鼠肉瘤被用于评估阿霉素对体内和体外肿瘤细胞的敏感性。两种肿瘤均经过组织培养适应，以便在体内或体外处理后能够在体外测定细胞存活率。对于这两种肿瘤（WHFIB和CBSAF），无论是通过细胞存活率还是生长延迟来测定，细胞在体外处理时高度敏感，但对体内处理却具有很强的抗性。两种肿瘤切除后立即用阿霉素在体外处理的细胞，比在体外培养的细胞略具抗性。然而，这不足以解释体内抗性和体外敏感性之间的显著差异。这种差异并非由于药物递送失败。细胞生长阶段是体外化学敏感性最显著的影响因素，平台期细胞比对数期细胞更具抗性。缺氧也是导致化学敏感性降低的一个重要因素。2至8毫米范围内的肿瘤直径似乎并不重要。
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