Roediger W E, Moore A
Dig Dis Sci. 1981 Feb;26(2):100-6. doi: 10.1007/BF01312224.
A method of perfusing the isolated human colon in vitro was developed to study the effect of the short-chain fatty acid n-butyrate on sodium absorption under controlled conditions. The isolated colon was viable in vitro provided that ischemia to the colon prior to perfusion was less than 40 min. Viability was judged on glucose utilization, mucosal potential difference, an sodium absorption. Sodium absorption from the lumen was observed either with or without 20 mM n-butyrate. In a control group sodium absorption (nmol/min/cm2 /+- SEM) was 320 /+- 10 (four perfusions, nine observation intervals) and potassium secretion 26 /+- 3 (four perfusions, nine observation intervals). With 20 mM n-butyrate sodium absorption was 1960 /+- 480 (four perfusions, ten observation intervals) (P less than 0.0025). Potassium secretion was 72 /+- 2 (four perfusions, ten observation intervals) and (P less than 0.025). Butyrate absorption was 254 /+- 60 (four perfusions, ten observation intervals) and correlated linearly with the unidirectional flux (Jm leads to S) of sodium (linear coefficient of 0.714, P = less than 0.001). These results suggest that the presence of bacterial short-chain fatty acids may determine the efficiency of sodium absorption in the colon and also indicate that an absence of short-chain fatty acids in the colon could be one factor leading to diminished sodium absorption in the colon of man.
为了在可控条件下研究短链脂肪酸正丁酸对钠吸收的影响，开发了一种体外灌注分离的人类结肠的方法。如果灌注前结肠的缺血时间少于40分钟，分离的结肠在体外是有活力的。通过葡萄糖利用、粘膜电位差和钠吸收来判断活力。观察有无20 mM正丁酸时肠腔的钠吸收情况。在对照组中，钠吸收（nmol/分钟/平方厘米±标准误）为320±10（四次灌注，九个观察间隔），钾分泌为26±3（四次灌注，九个观察间隔）。加入20 mM正丁酸后，钠吸收为1960±480（四次灌注，十个观察间隔）（P<0.0025）。钾分泌为72±2（四次灌注，十个观察间隔）（P<0.025）。丁酸吸收为254±60（四次灌注，十个观察间隔），且与钠的单向通量（Jm→S）呈线性相关（线性系数为0.714，P<0.001）。这些结果表明，细菌短链脂肪酸的存在可能决定结肠中钠吸收的效率，也表明结肠中缺乏短链脂肪酸可能是导致人类结肠钠吸收减少的一个因素。
