Wood J, Johnson R C, Palin K
J Clin Microbiol. 1981 Jan;13(1):102-5. doi: 10.1128/jcm.13.1.102-105.1981.
Observations of colonial growth of Leptospira interrogans serovar pomona, BJ, on soft-agar (1% agar) plates revealed the presence of both surface and subsurface colonies. The organisms from both types of colonies appeared to be similar in motility, morphology, antigenic composition, and pathogenicity. Passage in vivo tended to produce a higher incidence of surface colonies, whereas passage in vitro tended to produce a higher incidence of subsurface colonies, suggesting the importance of environmental factors in determining the type of colony produced.
对问号钩端螺旋体波摩那型BJ株在软琼脂（1%琼脂）平板上的菌落生长观察发现，既有表面菌落，也有深层菌落。两种菌落中的菌体在运动性、形态、抗原组成和致病性方面似乎相似。体内传代往往会产生较高比例的表面菌落，而体外传代则往往会产生较高比例的深层菌落，这表明环境因素在决定所产生菌落类型方面的重要性。
