Intervention study on the influence of reduction of occupational exposure to styrene on sister chromatid exchanges in lymphocytes.

An intervention study was performed on 28 workers exposed by inhalation to styrene in the reinforced plastics industry and 20 controls not occupationally exposed to the compound. The workers involved were 14 laminators exposed to a time-weighted average of approximately 40 ppm styrene and 14 formers exposed to an average of about 10 ppm styrene. Ambient air monitoring data and the concentration of mandelic acid in the urine were used for the assessment of exposure. From each subject, peripheral blood lymphocytes were analysed for sister chromatid exchanges (SCEs). In the laminators, the mean SCE frequency was significantly higher than in the controls in both the group of smokers (9.59 +/- 0.77 SCEs/cell vs 7.23 +/- 1.00 SCEs/cell) and the group of non-smokers (10.25 +/- 1.08 SCEs/cell vs 5.98 +/- 0.60 SCEs/cell). The mean SCE frequency of the formers (7.42 +/- 128 SCEs/cell in smokers) did not differ statistically from the controls (7.23 +/- 1.00 SCEs/cell in smokers). No evaluation was made for non-smoking formers since all but one worker in this group were smokers. In order to comply with a lowering of the occupational exposure limit (MAK value) for occupational exposure to styrene in the Federal Republic of Germany from 100 ppm to 20 ppm, considerable technical and hygienic improvements were made at the work site of the laminators. This intervention led to a reduction of average exposure of these workers by inhalation from 40 ppm to approximately 20 ppm. One year after these improvements were made, a second investigation was performed. In all but one of the laminators, the concentration of mandelic acid in urine had dropped considerably. The SCE frequency in blood lymphocytes of the laminators had likewise dropped significantly to 7.74 +/- 0.59 SCEs/cell in the non-smokers. In the smokers, it was also lower than on the first occasion (9.02 +/- 1.19), yet statistical evaluation was not possible due to insufficient numbers. Overall, the results of the intervention study show that the lowering of the occupational exposure limit for styrene to 20 ppm in Germany was justified and that a reduction of occupational exposure to the chemical has led to a prevention of adverse cytogenetic effects.