大鼠中的卵清蛋白免疫：通过酶联免疫吸附测定法同时检测IgM、IgG和IgE反应以及迟发型超敏反应。

Ovalbumin immunity in the rat: simultaneous testing of IgM, IgG and IgE response measured by ELISA and delayed-type hypersensitivity.

作者信息

Vos J G, Boerkamp J, Buys J, Steerenberg P A

出版信息

Scand J Immunol. 1980;12(4):289-95. doi: 10.1111/j.1365-3083.1980.tb00069.x.

DOI:10.1111/j.1365-3083.1980.tb00069.x

Abstract

After immunization of Wistar rats with different concentrations of ovalbumin (OA) emulsified in complete H37Ra adjuvant, the optimal antigen dose for specific IgM, IgG and IgE production and for delayed-type hypersensitivity appeared to be the same. It is therefore concluded that in the rat these function tests can be combined, which decreases the number of experimental animals needed. For the determination of IgM, IgG and IgE antibodies to OA, the enzyme-linked immunosorbent assay (ELISA) was used, which proved to be a specific method. In addition, using a multi-channel photometer, it is shown that specific antibody concentrations can be readily quantified by micro-ELISA instead of using end-point titrations.

摘要

用不同浓度的卵清蛋白（OA）乳化于完全H37Ra佐剂中对Wistar大鼠进行免疫后，产生特异性IgM、IgG和IgE以及迟发型超敏反应的最佳抗原剂量似乎相同。因此得出结论，在大鼠中这些功能测试可以合并进行，这减少了所需实验动物的数量。为了测定针对OA的IgM、IgG和IgE抗体，使用了酶联免疫吸附测定法（ELISA），该方法被证明是一种特异性方法。此外，使用多通道光度计表明，通过微ELISA可以很容易地定量特异性抗体浓度，而无需使用终点滴定法。